S01
Human tumor organoid biobanks for preclinical validation
Research Focus
The central project S01 will provide crucial support to all projects by establishing a shared repository of clinical samples and organoid cultures, along with corresponding clinical data. A comprehensive collection of primary CRC tumor tissue, metastases, and liquid biopsies will be prospectively gathered across all three sites. Single-cell RNA sequencing of both primary tumors and metastases will be conducted and integrated with spatial transcriptomic analysis (S02). A biobank of untransformed and tumor organoids will be created, accompanied by patient-derived stroma cells for functional assessment and validation.
Main Collaborations
- P01 Greten: Modulating CAF plasticity to enable immunotherapy of colorectal cancer:
- P03 Briquez/Fichtner-Feigl/Reuten: Metastasis predisposing extracellular matrix architecture in colorectal cancer:
- P04 Farin/Stemmler: Mapping the CAF subtype-dependent reciprocal signaling in the CRC niche:
- P05 Neufert: The role of LIFR signaling in CAFs in CRC:
- P06 Günther/Naschberger: Impact of vascular plasticity on therapy responses in CRC:
- P07 Berlin/Groß: Specific role(s) of the inflammasome in the TME of primary and metastasized sporadic CRC:
- P08 Weigert: The role of IL-38 in shaping tumor-promoting versus protective inflammation in colorectal cancer:
- P09 Koop/Neurath: Unraveling the Role of Stromal IL-36R Signaling in Colorectal Tumorigenesis:
- P10 Bengsch/Feuerstein: Targeting the intra-metastatic microbiome in colorectal cancer:
- P11 Arkan/Tatarova: Role of microbial amino acid metabolism on chemotherapy response in CRC:
- P13 Müller/Zundler: Modulation of CRC development and progression by GPR15L-dependent effects on lymphocyte infiltration and the intestinal microbiota:
- P15 Hildner: Tumor stroma-derived signals impair cDC1-dependent checkpoint inhibition as a potential resistance mechanism of colorectal cancer immunotherapy:
- P18 Buchholz/Farin: Identifying and overcoming CAR T cell barriers in the colorectal carcinoma microenvironment:
- P19 Wiegering: Targeting colorectal liver metastasis by overcoming MYC-induced immune evasion:
- S02 Reiss/Ritter: Spatial profiling of the tumor microenvironment in CRC:
- S03 Börries/Gupta: Research Information Infrastructure, Data Management and Bioinformatics Core:
Core Team
Prof. Dr. Florian Greten
Project Leader
Spokesperson
Georg-Speyer-Haus
