P10
Targeting the intra-metastatic microbiome in colorectal cancer
Project Leaders
Research Focus
Microbial induced signaling in circulation tumor cells (CTCs) primes the pre-metastatic niche in target organs. IP10will explore the molecular mechanisms regulating bacterial uptake at the primary tumor site and their subsequent release in the liver, utilizing murine models of metastatic CRC. Additionally, metastasis-associated microbiomes in human liver metastases across various CMS subtypes will be analyzed. Advanced techniques, including CyTOF and mass cytometry imaging, will elucidate the connections between the metastasis-associated microbiome, microbial-derived signals and metastasis development.
Main Collaborations
- P02 Büttner/Flinner: Multi modal prediction of therapy response for rectal cancer patients:
- P12 Rosshart: Identification of immunomodulatory microbial metabolites as novel therapeutics for advanced CRC:
- P16 Fichtner-Feigl/Imkeller: The role of primary-CRC-derived adaptive immune cells in anti-metastasis immunity:
- S01 Berlin/Greten/Naschberger: Human tumor organoid biobanks for preclinical validation:
- S02 Reiss/Ritter: Spatial profiling of the tumor microenvironment in CRC:
- S03 Börries/Gupta: Research Information Infrastructure, Data Management and Bioinformatics Core:
Core Team
Publications
- Single-cell deconvolution reveals high lineage- and location-dependent heterogeneity in mesenchymal multivisceral stage 4 colorectal cancer.
Berlin C, Mauerer B, Cauchy P, Luenstedt J, Sankowski R, Marx L, Feuerstein R, Schaefer L, Greten FR, Pesic M, Groß O, Prinz M, Ruehl N, Miketiuk L, Jauch D, Laessle C, Jud A, Biesel EA, Neeff H, Fichtner-Feigl S, Holzner PA, Kesselring R. J Clin Invest. 2023 Dec 28;134(5):e169576. doi: 10.1172/JCI169576. PMID: 38153787
- T-cell exhaustion and residency dynamics inform clinical outcomes in hepatocellular carcinoma.
Barsch M, Salié H, Schlaak AE, Zhang Z, Hess M, Mayer LS, Tauber C, Otto-Mora P, Ohtani T, Nilsson T, Wischer L, Winkler F, Manne S, Rech A, Schmitt-Graeff A, Bronsert P, Hofmann M, Neumann-Haefelin C, Boettler T, Fichtner-Feigl S, van Boemmel F, Berg T, Rimassa L, Di Tommaso L, Saeed A, D'Alessio A, Pinato DJ, Bettinger D, Binder H, John Wherry E, Schultheiss M, Thimme R, Bengsch B. J Hepatol. 2022 Aug;77(2):397-409. doi: 10.1016/j.jhep.2022.02.032. Epub 2022 Apr 1. PMID: 35367533
- Spatial single-cell profiling and neighbourhood analysis reveal the determinants of immune architecture connected to checkpoint inhibitor therapy outcome in hepatocellular carcinoma.
Salié H, Wischer L, D'Alessio A, Godbole I, Suo Y, Otto-Mora P, Beck J, Neumann O, Stenzinger A, Schirmacher P, Fulgenzi CAM, Blaumeiser A, Boerries M, Roehlen N, Schultheiß M, Hofmann M, Thimme R, Pinato DJ, Longerich T, Bengsch B. Gut. 2025 Feb 6;74(3):451-466. doi: 10.1136/gutjnl-2024-332837. PMID: 39349005